Contraction of glycerinated muscle fibers as a function of the ATP concentration
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Contraction of glycerinated muscle fibers as a function of the ATP concentration.
We have measured the force-velocity curves of glycerinated rabbit psoas fibers over a range of ATP concentration from 2.5 microM to 5 mM. As the ATP concentration is increased, the isometric tension increases to a maximum around 50 microM, then decreases to a plateau at 70% of the maximum by 1 mM ATP. At low ATP concentrations the maximum velocity of contraction is low and increases with increa...
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Observations were made on the Ca-sensitivity of glycerinated muscle bundles from dog intestine. The results obtained were as follows 1) The Ca-sensitivity of glycerinated muscle bundles depends on theduration of glycerol extraction. With long-term extraction, the muscle bundles lost a large part of the Ca-sensitivity of the ATP contractility. 2) When the bundles that had a typical Ca-sensitivit...
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The contraction of cardiac muscle that has been treated with glycerol requires Ca (pCa 8-5), when MgATP is used as a substrate. In contrast, this preparation contracts, even in the absence of Ca (pCa 8-10), when ATP is replaced by ITP. Ca dependency was not observed after Increasing free Ca concentrations from pCa 8.0 to 5.0, or after increasing MgTTP concentration from A to 80 mM. On the other...
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The contraction of cardiac muscle that has been treated with glycerol requires Ca2+ (pCa 8-5), when MgATP is used as a substrate. In contrast, this preparation contracts, even in the absence of Ca2+ (pCa 8-10), when ATP is replaced by ITP. Ca2+ dependency was not observed after increasing free Ca2+ concentrations from pCa 8.0 to 5.0, or after increasing MgITP concentration from 5 to 80 mM. On t...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1979
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(79)85174-7